2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Sustanzi standard użati fil-kurva tal-kalibrazzjoni tad-distribuzzjoni relattiva tal-massa molekulari: insulina, mikopeptidi, gliċina-gliċina-tirosina-arġinina, gliċina-gliċina-gliċina
3 Strument u tagħmir
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
B'mod ġenerali, il-proporzjon ta' aċidi amminiċi fil-prodotti ta' Sustar huwa ogħla minn dak fil-prodotti ta' Zinpro.
Parti 8 Effetti tal-użu
Effetti ta' sorsi differenti ta' traċċi ta' minerali fuq il-prestazzjoni tal-produzzjoni u l-kwalità tal-bajd tat-tiġieġ li jbidu fl-aħħar perjodu tal-bajd
Proċess ta' Produzzjoni
Teknoloġija ta' kelazzjoni mmirata
Teknoloġija ta' emulsifikazzjoni shear
Teknoloġija tal-isprej u t-tnixxif bil-pressjoni
Teknoloġija ta' refriġerazzjoni u deumidifikazzjoni
Teknoloġija avvanzata għall-kontroll ambjentali
Appendiċi A: Metodi għad-Determinazzjoni tad-distribuzzjoni relattiva tal-massa molekulari tal-peptidi
Adozzjoni tal-istandard: GB/T 22492-2008
1 Prinċipju tat-Test:
Dan ġie determinat permezz ta' kromatografija ta' filtrazzjoni bil-ġel ta' prestazzjoni għolja. Jiġifieri, bl-użu ta' mili poruż bħala fażi stazzjonarja, ibbażat fuq id-differenza fid-daqs tal-massa molekulari relattiva tal-komponenti tal-kampjun għas-separazzjoni, skoperta fil-bond peptidiku tat-tul ta' mewġa ta' assorbiment ultravjola ta' 220nm, bl-użu tas-softwer iddedikat għall-ipproċessar tad-dejta għad-determinazzjoni tad-distribuzzjoni relattiva tal-massa molekulari permezz tal-kromatografija ta' filtrazzjoni bil-ġel (jiġifieri, is-softwer GPC), il-kromatogrammi u d-dejta tagħhom ġew ipproċessati, ikkalkulati biex jinkiseb id-daqs tal-massa molekulari relattiva tal-peptid tas-sojja u l-firxa tad-distribuzzjoni.
2. Reaġenti
L-ilma sperimentali għandu jissodisfa l-ispeċifikazzjoni tal-ilma sekondarju f'GB/T6682, l-użu ta' reaġenti, ħlief għal dispożizzjonijiet speċjali, għandu jkun analitikament pur.
2.1 Ir-reaġenti jinkludu aċetonitrile (kromatografikament pur), aċidu trifluworoaċetiku (kromatografikament pur),
2.2 Sustanzi standard użati fil-kurva tal-kalibrazzjoni tad-distribuzzjoni relattiva tal-massa molekulari: insulina, mikopeptidi, gliċina-gliċina-tirosina-arġinina, gliċina-gliċina-gliċina
3 Strument u tagħmir
3.1 Kromatografu Likwidu ta' Prestazzjoni Għolja (HPLC): stazzjon tax-xogħol kromatografiku jew integratur b'ditekter UV u softwer tal-ipproċessar tad-dejta GPC.
3.2 Unità ta' filtrazzjoni u tneħħija tal-gass bil-vakwu f'fażi mobbli.
3.3 Bilanċ elettroniku: valur gradwat 0.000 1g.
4 Passi operattivi
4.1 Kundizzjonijiet kromatografiċi u esperimenti ta' adattament tas-sistema (kundizzjonijiet ta' referenza)
- 4.1.1 Kolonna kromatografika: TSKgelG2000swxl300 mm × 7.8 mm (dijametru intern) jew kolonni oħra tal-ġell tal-istess tip b'prestazzjoni simili adattati għad-determinazzjoni ta' proteini u peptidi.
- 4.1.2 Fażi mobbli: Aċetonitrile + ilma + aċidu trifluworoaċetiku = 20 + 80 + 0.1.
- 4.1.3 Tul ta' mewġa tad-detezzjoni: 220 nm.
- 4.1.4 Rata tal-fluss: 0.5 mL/min.
- 4.1.5 Ħin ta' skoperta: 30 minuta.
- 4.1.6 Volum tal-injezzjoni tal-kampjun: 20μL.
- 4.1.7 Temperatura tal-kolonna: temperatura tal-kamra.
- 4.1.8 Sabiex is-sistema kromatografika tissodisfa r-rekwiżiti ta' skoperta, ġie stipulat li taħt il-kundizzjonijiet kromatografiċi ta' hawn fuq, l-effiċjenza tal-kolonna kromatografika tal-ġel, jiġifieri, in-numru teoretiku ta' pjanċi (N), ma tkunx inqas minn 10000 ikkalkulata abbażi tal-qċaċet tal-istandard tat-tripeptide (Glycine-Glycine-Glycine).
- 4.2 Produzzjoni ta' kurvi standard tal-massa molekulari relattiva
- Is-soluzzjonijiet standard ta' peptidi b'massa molekulari relattiva differenti msemmija hawn fuq b'konċentrazzjoni ta' massa ta' 1 mg/mL ġew ippreparati permezz ta' tqabbil tal-fażi mobbli, imħallta f'ċertu proporzjon, u mbagħad iffiltrati minn membrana ta' fażi organika b'daqs tal-pori ta' 0.2 μm~0.5 μm u injettati fil-kampjun, u mbagħad inkisbu l-kromatogrammi tal-istandards. Il-kurvi ta' kalibrazzjoni tal-massa molekulari relattiva u l-ekwazzjonijiet tagħhom inkisbu billi ġie pplottjat il-logaritmu tal-massa molekulari relattiva kontra l-ħin ta' żamma jew permezz ta' rigressjoni lineari.
4.3 Trattament tal-kampjun
Iżen b'mod preċiż 10mg tal-kampjun fi flask volumetriku ta' 10mL, żid ftit fażi mobbli, ħawwad ultrasoniku għal 10min, sabiex il-kampjun jinħall kompletament u jitħallat, jiġi dilwit mal-fażi mobbli sal-iskala, u mbagħad iffiltra minn membrana tal-fażi organika b'daqs tal-pori ta' 0.2μm ~ 0.5μm, u l-filtrat ġie analizzat skont il-kundizzjonijiet kromatografiċi f'A.4.1.
- 5. Kalkolu tad-distribuzzjoni relattiva tal-massa molekulari
- Wara li tiġi analizzata s-soluzzjoni tal-kampjun ippreparata f'4.3 taħt il-kundizzjonijiet kromatografiċi ta' 4.1, il-massa molekulari relattiva tal-kampjun u l-firxa tad-distribuzzjoni tiegħu jistgħu jinkisbu billi d-dejta kromatografika tal-kampjun tiġi sostitwita fil-kurva tal-kalibrazzjoni 4.2 b'softwer tal-ipproċessar tad-dejta GPC. Id-distribuzzjoni tal-mases molekulari relattivi tal-peptidi differenti tista' tiġi kkalkulata bil-metodu ta' normalizzazzjoni tal-erja tal-quċċata, skont il-formula: X=A/A total×100
- Fil-formula: X - Il-frazzjoni tal-massa ta' peptid b'massa molekulari relattiva fil-peptid totali fil-kampjun, %;
- A - Żona tal-quċċata ta' peptide b'massa molekulari relattiva;
- Total A - is-somma tal-erjas tal-quċċata ta' kull peptide b'massa molekulari relattiva, ikkalkulata sa post deċimali wieħed.
- 6 Ripetibbiltà
- Id-differenza assoluta bejn żewġ determinazzjonijiet indipendenti miksuba taħt kundizzjonijiet ta' ripetibbiltà m'għandhiex taqbeż il-15% tal-medja aritmetika taż-żewġ determinazzjonijiet.
- Appendiċi B: Metodi għad-Determinazzjoni tal-Aċidi Amino Ħielsa
- Adozzjoni tal-istandard: Q/320205 KAVN05-2016
- 1.2 Reaġenti u materjali
- Aċidu aċetiku glaċjali: analitikament pur
- Aċidu perkloriku: 0.0500 mol/L
- Indikatur: 0.1% indikatur kristall vjola (aċidu aċetiku glaċjali)
- 2. Determinazzjoni ta' aċidi amminiċi ħielsa
Il-kampjuni tnixxfu f'temperatura ta' 80°C għal siegħa.
Poġġi l-kampjun f'kontenitur niexef biex jiksaħ b'mod naturali għat-temperatura tal-kamra jew jiksaħ għal temperatura użabbli.Iżen madwar 0.1 g tal-kampjun (preċiż sa 0.001 g) ġo flask koniku niexef ta' 250 mL.Ipproċedi malajr għall-pass li jmiss biex tevita li l-kampjun jassorbi l-umdità ambjentaliŻid 25 mL ta' aċidu aċetiku glaċjali u ħawwad sew għal mhux aktar minn 5 minuti.Żid żewġ qatriet ta' indikatur kristall vjolaIttitra b'soluzzjoni standard ta' titrazzjoni ta' 0.0500 mol / L (±0.001) ta' aċidu perkloriku sakemm is-soluzzjoni tinbidel minn vjola għall-punt finali.
Irreġistra l-volum tas-soluzzjoni standard ikkonsmata.
- Agħmel it-test vojt fl-istess ħin.
- 3. Kalkolu u riżultati
- Il-kontenut ta' amino acid ħieles X fir-reaġent huwa espress bħala frazzjoni tal-massa (%) u huwa kkalkulat skont il-formula: X = C × (V1-V0) × 0.1445/M × 100%, fil-formula:
- C - Konċentrazzjoni ta' soluzzjoni standard ta' aċidu perkloriku f'moli kull litru (mol/L)
- V1 - Volum użat għat-titrazzjoni tal-kampjuni b'soluzzjoni standard ta' aċidu perkloriku, f'millilitri (mL).
- Vo - Volum użat għall-vojt tat-titrazzjoni b'soluzzjoni standard ta' aċidu perkloriku, f'millilitri (mL);
M - Massa tal-kampjun, fi grammi (g).
| 0.1445: Massa medja ta' aċidi amminiċi ekwivalenti għal 1.00 mL ta' soluzzjoni standard ta' aċidu perkloriku [c(HClO4) = 1.000 mol / L]. | 4.2.3 Soluzzjoni standard ta' titrazzjoni tas-sulfat taċ-ċerju: konċentrazzjoni c [Ce (SO4) 2] = 0.1 mol/L, ippreparata skont GB/T601. | |
| Adozzjoni ta' standards: Q/70920556 71-2024 | 1. Prinċipju tad-determinazzjoni (Fe bħala eżempju) | Il-kumplessjonijiet tal-ħadid tal-aċidi amminiċi għandhom solubilità baxxa ħafna fl-etanol anidru u l-joni tal-metall ħielsa jinħallu fl-etanol anidru, id-differenza fis-solubilità bejn it-tnejn fl-etanol anidru ntużat biex tiddetermina r-rata ta' kelazzjoni tal-kumplessjonijiet tal-ħadid tal-aċidi amminiċi. |
| Fil-formula: V1 - volum tas-soluzzjoni standard tas-sulfat taċ-ċerju kkunsmat għat-titrazzjoni tas-soluzzjoni tat-test, mL; | Etanol anidru; il-bqija huwa l-istess bħall-klawżola 4.5.2 fil-GB/T 27983-2011. | 3. Passi tal-analiżi |
| Agħmel żewġ provi b'mod parallel. Iżen 0.1g tal-kampjun imnixxef f'103±2℃ għal siegħa, b'preċiżjoni ta' 0.0001g, żid 100mL ta' etanol anidru biex jinħall, iffiltra, iffiltra r-residwu maħsul b'100mL ta' etanol anidru għal mill-inqas tliet darbiet, imbagħad ittrasferixxi r-residwu ġo flask koniku ta' 250mL, żid 10mL ta' soluzzjoni ta' aċidu sulfuriku skont il-klawżola 4.5.3 f'GB/T27983-2011, u mbagħad wettaq il-passi li ġejjin skont il-klawżola 4.5.3 "Saħħan biex jinħall u mbagħad ħalli jiksaħ" f'GB/T27983-2011. Wettaq it-test vojt fl-istess ħin. | 4. Determinazzjoni tal-kontenut totali tal-ħadid | 4.1 Il-prinċipju tad-determinazzjoni huwa l-istess bħal dak tal-klawżola 4.4.1 fil-GB/T 21996-2008. |
4.2. Reaġenti u Soluzzjonijiet
| 4.2.1 Aċidu mħallat: Żid 150mL ta' aċidu sulfuriku u 150mL ta' aċidu fosforiku ma' 700mL ta' ilma u ħawwad sew. | 4.2.2 Soluzzjoni indikatur tas-sodium diphenylamine sulfonate: 5g/L, ippreparata skont GB/T603. | 4.2.3 Soluzzjoni standard ta' titrazzjoni tas-sulfat taċ-ċerju: konċentrazzjoni c [Ce (SO4) 2] = 0.1 mol/L, ippreparata skont GB/T601. | |
| 4.3 Passi tal-analiżi | Agħmel żewġ provi b'mod parallel. Iżen 0.1g tal-kampjun, preċiż sa 020001g, poġġih fi flask koniku ta' 250mL, żid 10mL ta' aċidu mħallat, wara d-dissoluzzjoni, żid 30ml ilma u 4 qatriet ta' soluzzjoni tal-indikatur tas-sodium dianiline sulfonate, u mbagħad wettaq il-passi li ġejjin skont il-klawżola 4.4.2 f'GB/T21996-2008. Wettaq it-test vojt fl-istess ħin. | 4.4 Rappreżentazzjoni tar-riżultati | Il-kontenut totali ta' ħadid X1 tal-kumplessjonijiet tal-ħadid tal-aċidi amminiċi f'termini ta' frazzjoni tal-massa tal-ħadid, il-valur espress f'%, ġie kkalkulat skont il-formula (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - soluzzjoni standard tas-sulfat taċ-ċerju kkunsmata għat-titrazzjoni tas-soluzzjoni vojta, mL; | V0 - soluzzjoni standard tas-sulfat taċ-ċerju kkunsmata għat-titrazzjoni tas-soluzzjoni vojta, mL; | C - Konċentrazzjoni attwali tas-soluzzjoni standard tas-sulfat taċ-ċerju, mol/L5. Kalkolu tal-kontenut tal-ħadid fil-kelatiIl-kontenut tal-ħadid X2 fil-kelat f'termini tal-frazzjoni tal-massa tal-ħadid, il-valur espress f'%, ġie kkalkulat skont il-formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Fil-formula: V1 - volum tas-soluzzjoni standard tas-sulfat taċ-ċerju kkunsmat għat-titrazzjoni tas-soluzzjoni tat-test, mL; | V2 - soluzzjoni standard tas-sulfat taċ-ċerju kkunsmata għat-titrazzjoni tas-soluzzjoni vojta, mL;nom1-Massa tal-kampjun, g. Ħu l-medja aritmetika tar-riżultati tad-determinazzjoni parallela bħala r-riżultati tad-determinazzjoni, u d-differenza assoluta tar-riżultati tad-determinazzjoni parallela ma tkunx aktar minn 0.3%. | 0.05585 - massa ta' ħadid ferruż espressa fi grammi ekwivalenti għal 1.00 mL ta' soluzzjoni standard ta' sulfat taċ-ċerju C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-Massa tal-kampjun, g. Ħu l-medja aritmetika tar-riżultati tad-determinazzjoni parallela bħala r-riżultati tad-determinazzjoni, u d-differenza assoluta tar-riżultati tad-determinazzjoni parallela ma tkunx aktar minn 0.3%. | 6. Kalkolu tar-rata ta' kelazzjoniIr-rata ta' kelazzjoni X3, il-valur espress f'%, X3 = X2/X1 × 100Appendiċi Ċ: Metodi għad-Determinazzjoni tar-rata ta' kelazzjoni ta' Zinpro |
Adozzjoni tal-istandard: Q/320205 KAVNO7-2016
1. Reaġenti u materjali
a) Aċidu aċetiku glaċjali: analitikament pur; b) Aċidu perkloriku: 0.0500mol/L; ċ) Indikatur: 0.1% indikatur kristall vjola (aċidu aċetiku glaċjali)
2. Determinazzjoni ta' aċidi amminiċi ħielsa
2.1 Il-kampjuni tnixxfu f'temperatura ta' 80°C għal siegħa.
2.2 Poġġi l-kampjun f'kontenitur niexef biex jiksaħ b'mod naturali għat-temperatura tal-kamra jew jiksaħ għal temperatura użabbli.
2.3 Iżen madwar 0.1 g tal-kampjun (preċiż sa 0.001 g) ġo flask koniku niexef ta' 250 mL
2.4 Ipproċedi malajr għall-pass li jmiss biex tevita li l-kampjun jassorbi l-umdità ambjentali.
2.5 Żid 25mL ta' aċidu aċetiku glaċjali u ħawwad sew għal mhux aktar minn 5 minuti.
2.6 Żid 2 qatriet ta' indikatur kristall vjola.
2.7 Ittitra b'soluzzjoni standard ta' titrazzjoni ta' 0.0500mol/L (±0.001) ta' aċidu perkloriku sakemm is-soluzzjoni tinbidel minn vjola għal ħadra għal 15-il sekonda mingħajr ma tbiddel il-kulur bħala l-punt finali.
2.8 Irreġistra l-volum tas-soluzzjoni standard ikkonsmata.
2.9 Agħmel it-test vojt fl-istess ħin.
- 3. Kalkolu u riżultati
- Katalan
- Physicochemical parameters
V1 - Volum użat għat-titrazzjoni tal-kampjuni b'soluzzjoni standard ta' aċidu perkloriku, f'millilitri (mL).
Vo - Volum użat għall-vojt tat-titrazzjoni b'soluzzjoni standard ta' aċidu perkloriku, f'millilitri (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Indirizz: Nru. 147 Triq Qingpu, Belt Shouan, Kontea ta' Pujiang, Belt Chengdu, Provinċja ta' Sichuan, iċ-Ċina
Telefon: 86-18880477902
Prodotti
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- Minerali organiċi traċċi
- Swaħili
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- Konnessjonijiet malajr
Profil tal-Kumpanija
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Guġarati | Ikklikkja għal inkjesta | © Drittijiet tal-Awtur - 2010-2025: Id-Drittijiet kollha Riżervati. | Mappa tas-sit L-AQWA TIFTXA Telefon |
| Tel | 86-18880477902 | Ġavaniż | E-mail |
| 8618880477902 | Ċiniż | Franċiż | |
| Bird | Ċiniż | Franċiż | Ġermaniż Spanjol |
| Aquatic animals | Ġappuniż | Korean | Għarbi Grieg |
| Tork | Taljan | ||
| Ruminant animal g/head day | January 0.75 | Indoneżjan Afrikans Żvediż |
Pollakk
- Bask
- Katalan
- Physicochemical parameters
Ħindi
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Xona
Bulgaru
- Ċebujan
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Kroat
Olandiż
| Application object | Urdu Vjetnamiż | Content in full-value feed (mg/kg) | Efficacy |
| Guġarati | Ħajtin | Ħawsa | Kinjarwanda Ħmong Ungeriż |
| Piglets and fattening pigs | Igbo | Ġavaniż | Kannada Khmer Kurd |
| Kirgiż | Latin | ||
| Bird | 300～400 | 45～60 | Maċedonjan Malajan Malajalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norveġiż
- Paxto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serb
Sesoto
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Xona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swaħili
Taġik
Tamil
Telugu
Tajlandiż
| Application object | Urdu Vjetnamiż | Content in full-value feed (mg/kg) | Efficacy |
| Jiddix | Joruba | Żulu | Kinjarwanda Orija Turkmen |
| Ujgur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
